达州脱毒马铃薯种薯繁育技术规程 Technicalregulationofvirusfreeseedpotatopropagationin DazhouDB5117 四川省（达州市）地方标准 DB5117/T73-2023 2023-04-25发布 达州市市场监督管理局发布2023-05-01实施ICS03.080.01 CCSA16 DB5117/T73-2023 I目次 目次·····························································································································································Ⅰ 前言·····························································································································································Ⅱ 1范围·························································································································································1 2规范性引用文件·····································································································································1 3术语与定义·············································································································································1 4技术要求·················································································································································1 5病虫害防治·············································································································································4 附录A（规范性）MS培养基配方·········································································································5 附录B（规范性）雾培营养液配方·······································································································6 附录C（资料性）达州地区常见马铃薯病虫害防治···········································································7 附录C（资料性）续达州地区常见马铃薯病虫害防治·······································································8 参考文献·····················································································································································9 DB5117/T73-2023 II前  言 本文件按照GB/T1.1-2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定 起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。 本文件由达州市农业科学研究院提出。 本文件由达州市农业农村局归口。 本文件起草单位：达州市农业科学研究院。 本文件主要起草人：高龙梅、吴明阳、李益、杨小丽、张玲、贾荟芹、刘小英、李薇、高川、范香 全、赵思毅、周娜交。 DB5117/T73-2023 1达州脱毒马铃薯种薯繁育技术规程 1范围 本文件规定了达州脱毒马铃薯种薯繁育技术要求。 本文件适用于达州市行政区域内脱毒马铃薯种薯繁育。 2规范性引用文件 本文件没有规范性引用文件。 3术语与定义 本文件没有需要界定的术语和定义。 4技术要求 4.1组培苗繁育 4.1.1实验室要求 脱毒马铃薯组培实验室包括：药品室、洗涤室、准备室、接种室、培养室。配备完善的水、电供应 系统和空气、灯光、温度、湿度调节系统。 4.1.2培养基的配制 4.1.2.1母液配制 将培养基所需的大量元素、铁盐、有机成分配制成100倍使用浓度的母液，微量元素配制成1000 倍使用浓度的母液。母液配制应符合附录A的规定。 4.1.2.2MS培养基配制 在水中加入琼脂熬至清澈后，按比例添加附录A中的四种母液，再加入白砂糖或蔗糖，糖浓度为 20g/L～30g/L，琼脂粉浓度为5g/L～7g/L。 4.1.2.3培养基配方 脱毒配方：MS+6-BA0.1mg/L+NAA0.05mg/L+GA30.1mg/L 快繁配方：MS 4.1.2.4培养基pH调节 用稀盐酸或氢氧化钾溶液调节培养基pH值到5.70～5.75。 4.1.2.5培养基分装和灭菌 配制好的培养基趁热分装，培养基厚度1.5cm～2.5cm。分装后立即加盖或用封口膜封严。分装后24h 内进行高压灭菌处理，在0.8kPa～1.1kPa、121℃状态下保持25min。