ISO INTERNATIONAL STANDARD 5667-16 Secondedition 2017-04 Water quality - Sampling Part 16: Guidance on biotesting of samples Qualité de I'eau- Echantillonnage - Partie 16: Lignes directrices pour les essais biologiques des échantillons Reference number IS0 5667-16:2017(E) International Organization for Standardization @ IS0 2017 e=ZHEJIANG INST OF STANDARDIZATION C1 5956617 nitted without license from IHS IS0 5667-16:2017(E) COPYRIGHT PROTECTEDDOCUMENT IS0 2017, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyright@iso.org www.iso.org Licensee-ZHEJIANG INST OF STANDARDIZAISQ 017 - All rights reserVed Not for Resale, 2017/6/28 01:00:27 networking permited without license from IHS IS0 5667-16:2017(E) Contents Page Foreword ..V Introduction. ..vi 1 Scope. 2 Normative references 3 Terms and definitions 4 General guidance regarding test design ..5 4.1 General. .5 .5 4.2 Replicates. 4.2.1 General. ..5 4.2.2 Lowest ineffective dilution (LID) 4.2.3 Hypothesis testing - two-sample comparisons .6 4.2.4 Concentration and dilution response relationship ..6 5 Evaluation 5.1 General. 7 5.2 Statistical analysis. .7 Sampling and transportation 1 6 6.1 General 7 6.2 Sampling equipment. ..8 6.2.1 General. ..8 6.2.2 Sample container .8 6.3 Filling status of sample containers .9 6.4 Sample identification and records .9 6.5 Sub-sampling. 6.6 Transportation ..10 6.7 Contamination during sampling ..10 6.8 Sampling quality control techniques .10 7 Pre-treatment. ..11 7.1 General. 11 7.2 Preservation and storage .11 7.3 Thawing ..12 7.4 Homogenization ..12 7.5 Separation of soluble and particulate matter ..12 7.6 Preconcentration. ..13 7.6.1 General. .13 7.6.2 Extraction methods ..14 7.7 pH adjustment. ..14 8 Apparatus and equipment ..14 8.1 Selection of apparatus ..14 8.2 Cleaning of apparatus and equipment. ..15 9 Impairment of test performance .15 9.1 Problems and preventive measures for samples containing removable ingredients .15 9.1.1 General. .15 9.1.2 Volatilization. .16 9.1.3 Foaming ..16 9.1.4 Adsorption. ..16 9.1.5 Precipitation/flocculation ..16 9.1.6 Degradation ..16 9.2 Problems and preventive measures concerning coloured and/or turbid samples ..17 10 Preparation of stock solutions and test batches .17 10.1 Water-soluble substances .17 ntemaonalgnzatin7-All rights reserved iii icensee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 vided by IHS under license with ISO d without license from IHS Not for Resale, 2017/6/28 01:00:27