ISO INTERNATIONAL STANDARD 8784-1 Third edition 2014-12-01 Pulp, paper and board . Microbiological examination - Part 1: Enumeration of bacteria and bacterial spores based on disintegration Pates, papiers et cartons -Analyse microbienne - Partie 1: Denombrement des bacteries et des spores bacteriennes basé sur la désintégration Reference number IS0 8784-1:2014(E) SO International Organization for Standardization @ IS0 2014 dardization 5956617 ed without license from IHS IS0 8784-1:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ ISO 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland Prganizationfor Standardization Not for Resale, 2015/3/20 05:27:36 etworking permitted without license from IHS IS0 8784-1:2014(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Culture media and diluents .2 5.1 General. .2 5.2 Water. .2 5.3 Culture media for total bacteria count and spore count .2 5.4 Diluents .2 Apparatus and equipment. .3 6 6.1 General 3 6.2 List of equipment .3 7 Sampling. ..3 8 Preparation of the test material ..4 8.1 General .4 8.2 Determination of dry-matter content 8.3 Weighing 4 8.4 Disintegration 4 9 Determination of the total bacterial count and spore count .5 9.1 General .5 9.2 Plating for total bacterial count ..5 9.3 Plating for spore count .6 9.4 Incubation. .6 10 Enumeration of the colonies ..6 11 Calculation and report ..6 11.1 Calculation . 6 11.2 Interpretation ..7 11.3 Report ..8 12 Testreport ..8 Annex A (informative) Dilution fluid Annex B (informative) Precision. ..10 Bibliography ..14 iii e=Zhejiang Institute of Standardization 5956617 vided by IHS underI d without license from IHS