ISO INTERNATIONAL STANDARD 9936 Third edition 2016-04-01 Animal and vegetable fats and oils Determination of tocopherol and tocotrienol contents by high- performance liquid chromatography Corps gras d'origines animale et vegétale Détermination des teneurs en tocopherols et en tocotriénols par chromatographie en phase liquide a haute performance Reference number IS0 9936:2016(E) ISO International Organization for Standardization @ IS0 2016 vided by IHS under ed without license from IHS IS0 9936:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS02016,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax+412274909 47 copyright@iso.org www.iso.org Intenationai br PrganizationforStandardization icensee-ZHEJIANG INST OF STANDARDIZoISQ s.6 - All rights reserved I by IHS unde etworking permitted without license from IHS IS0 9936:2016(E) Contents Page Foreword ..iv 1 Scope 2 Normative references ..1 3 Terms and definitions 4 Principle 5 Reagents. ..1 6 Apparatus. .2 7 Sampling. ..3 8 Preparation of test sample .3: 9 Procedure .3 9.1 Preparation of calibration solutions .4: 9.1.1 Stockcalibrationsolutions 4 9.1.2 Standard solution. .4 9.2 Optimization of working parameters 4 9.3 Preparation of test solution .5 9.4 Determination .5 10 Expression of results ..6 11 Precision .6 11.1 Interlaboratory test .6 11.2 Repeatability .6 11.3 Reproducibility .7 12 Test report ..7 Annex A (informative) Examples of chromatograms ..8 Annex B (informative) Saponification ..10 Annex C (informative) Results of interlaboratory tests ..12 Bibliography .16 iii e=ZHEJIANG INSTOFSTANDARDIZATION C15956617 vided by IHS under lic ithout license from IHS