ISO INTERNATIONAL STANDARD 11269-2 Third edition 2012-01-15 Soil quality Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants Qualite du sol- Détermination des effets des polluants sur la flore du sol- Partie 2: Effets des sols contaminés sur I'émergence et la croissance des végétaux supérieurs Reference number ISO 11269-2:2012(E) ISO @ISO2012 itted without license from IHS Not for Resale ISO 11269-2:2012(E) COPYRiGHTPROTECTEDDOCUMENT ISO 2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland ISO 2012-All rights reserved or networking permited withoutlicense from IHS Not for Resale ISO 11269-2:2012(E) Contents Page Foreword ..iv Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Units 5 Principle. 3 6 Test plants. 3 7 Materials. 7.1 Testyessels 7.2 Soil. 8 Equipment .6 9 Reference substance 10 Procedure. 10.1 Experimental design. 10.2 Preparation of the pots. 10.3 Preparation of the seeds. 10.4 Growth conditions.. 10.5 Start of the test. 10.6 Handling during the test. 8 11 Validity criteria 9 12 Assessmentof the results 12.1 Data presentation... 12.2 Expression of the results . 9 13 Statistical analysis 9 13.1 General. 13.2 Range-finding test. 13.3 Final test .. 10 14 Test report. .11 Annex A (informative) Additional recommended plant species based on test results gained by applying Environment Canada Test Method: EPs 1/RM/45[4] .... Annex B (informative) Phytotoxic values for reference compounds: sodium trichloro-acetate and boric acid. ..15 Annex C (informative) Recommended method for the measuring of the water-holding capacity of the soil... 16 Annex D (informative) Recommendations for nutrient supply of soils.. 17 Bibliography. 18 ili ed without license from IHS Not for Resale