ISO INTERNATIONAL STANDARD 11350 First edition 2012-05-15 Water quality Determination of the genotoxicity of water and waste water - Salmonella/microsome fluctuation test (Ames fluctuation test) Qualite de I'eau - Evaluation de la génotoxicité des eaux résiduaires - Essai de Salmonella/microsome (essai d'Ames-fluctuation) Reference number ISO 11350:2012(E) ISO @ISO2012 No reproduction or networking permitted without license from IHS Not for Resale ISO11350:2012(E) COPYRiGHTPROTECTEDDOCUMENT ISO2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ ISO 2012 - All rights reserved or networking permited withoutlicense from IHS Not for Resale ISO 11350:2012(E) Contents Page Foreword ..iv 1 Scope 2 Normative references 3 Terms and definitions. 2 4 Interference 5 Principle. 6 Apparatus and materials 7 Reagents, media and dilutions 5 8 Sampling and samples. .9 9 Procedure 9 9.1 Overnight culture. 9 9.2 Preparation of S9 mix. 10 9.3 Testing of water samples 10 9.4 Measurement of revertant growth. 13 9.5 Calculation of cytotoxicity 13 10 Validity criteria 14 11 Assessment criteria 14 12 Test report 14 Annex A (normative) Nutrient broth and agar. .15 Annex B (normative) Preparation of ampicillin agar plates and stock cultures. 16 Annex C (normative) Checking of genotype, 17 Annex D (normative) S9 fraction.. .18 Annex E (informative) Example for application of samples on a 24 well plate. .19 Annex F (informative) Example for reporting... .21 Annex G (informative) Testing of chemicals 22 Annex H (informative) Precision data.. .25 Annex I (informative) Statistical assessment. Annex J (informative) Measurement of the lowest ineffective dilution (LiD) of a waste water A simplified evaluation for testing of waste water... .33 Annex K (informative) Use of additional tester strains .35 Bibliography 36 ili lout license from IHS Not for Resale