ISO INTERNATIONAL STANDARD 13629-1 First edition 2012-08-15 Textiles Determination of antifungal activity of textile products - Part 1: Luminescence method Textiles-Determinationde I'activite antifongique des produits textiles- Partie 1:Methode parluminescence Reference number ISO 13629-1:2012(E) TSO @ISO2012 ISO 13629-1:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO2012 ? All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 · CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland = @ ISO 2012 -All rights reserved ISO 13629-1:2012(E) Contents Page Foreword .. iv Introduction 1 Scope. 2 Normative reference 3 Terms and definitions 4 Principle 5 Safety precaution 6 Reference fungi.. 2 7 Apparatus 2 8 Reagents and culture media.. 3 8.1 General 3 8.2 Purewater 8.3 Anionic surfactant. 8.4 Luminescent reagents, reagents and buffer solutions 4 8.5 Culture medium 9 Fungus preservation and use 6 9.1 Fungus preservation 6 9.2 Use of fungus 10 Spore suspension 10.1 Suspending spores in culture media. 8 10.2 Collection and dispersion of spore suspension froma culture medium. 8 10.3 Filtering to remove hyphae and spore threads. 8 10.4 Using centrifugal separation and resuspension to remove supernatant 8 10.5 Confirming the concentration of spore suspension.. 9 10.6 Adjusting spore suspension for testing... 9 11 Preparing the ATP calibration curve 9 12 Testing method 10 12.1 Preparation of test specimens and inoculation 10 12.2 Incubation.. 12 13 Measurement of luminescence intensity 12 13.1 Absorption method. 12 13.2 Transfer method. .13 14 Calculation 14 14.1 Judgment of test effectiveness .. 14 14.2 Calculation of antifungal activity value... ..14 15 Test report. 15 Annex A (normative) The fungi used in this part of Iso 13629 .16 Annex B (informative) Antifungal efficacy . 17 Bibliography @ ISO 2012 - All rights reserved ii