ISO/TS TECHNICAL SPECIFICATION 14101 Firstedition 2012-11-01 Surface characterization of gold nanoparticles for nanomaterial specific toxicity screening: FT-IR method Caracterisation de surface des nanoparticulesd'or pour criblage de toxicité spécifiquedenanomatériau:methodeFT-IR Reference number ISO/TS14101:2012(E) ISO @IS0 2012 IS0/TS 14101:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in anyform or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either Iso at the address below or Iso's memberbodyin thecountry of the requester. ISO copyright office Case postale 56.CH-1211 Geneva 20 Tel. +4122 749 0111 Fax+41227490947 E-mail [email protected] Web www.iso.org Published in Switzerland 1 @ IS0 2012 - All rights reserved IS0/TS14101:2012(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normativereferences .1 3 Terms and definitions .1 4 Symbolsandabbreviatedterms .3 5 Sample preparation mode .3 5.1 Removal of unbound molecules 3 5.2 Dehydration .6 5.3 Screening test for impurities in DW from sample tubes 6 6 FT-IRmeasurementprocedure .7 6.1 General .7 6.2 ATRmethod .7 6.3 Transmission method .8 6.4 Determination of time required for completepurge 9 6.5 Linear range of IR band intensity versus concentration .10 6.6 LODand LOQdetermination .11 6.7 Repeatability determination ..12 7 Application examples .12 7.1 Degrees of ligand exchange .12 7.2 Qualitativemeasurementof biomolecularbinding ..13 AnnexA(informative)Casestudyforvalidationofligandexchange 15 AnnexB (informative)Case studyforqualitativemeasurementofbiochemical moietiesbindingto theAuNPsurface 17 AnnexC (informative)Selectionguideforwindowmaterials .20 Bibliography .21 @s?Qll rights reserved iii