ISO INTERNATIONAL STANDARD 15214 First edition 1998-08-01 Microbiology of food and animal feeding stuffs Horizontal method for the emumeration of mesophilic lactic acid bacteria Colony-count technique at 3o °C Microbiologie des aliments - Methode horizontale pour le denombrement des bactéries lactiques meésophiles Techniques par comptage des colonies a 30 ℃ ISO Referencenumber ISO 15214:1998(E) HS under I without license from IHS Not for Resale ISO 15214:1998(E) Contents Page Scope 1 2 Normative references 1 3 Definitions 1 4 Principle 2 5 Diluent and culture medium 2 6 Apparatus and glassware 3 7 Sampling 3 8 Preparation of test sample 4 9 Procedure 10 Expression of results 5 11 Confidence limits 9 12 Test report 9 Annex A (informative) Bibliography 7 @ISO1998 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International OrganizationforStandardization Case postale 56 . CH-1211 Geneve 20 · Switzerland Internet [email protected] Printed in Switzerland Copyright International Organization for Standardization vided by IHS under lic Not for Resale ISO 15214:1998(E) @ ISO Foreword IsO (the International Organization for Standardization) is a worldwide federationofnationalstandardsbodies(lSOmemberbodies).Theworkof preparing International Standards is normally carried out through isO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non- governmental, in liaison with ISo, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (lEC)onallmattersofelectrotechnicalstandardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. ISO/TC 34, Agricultural food products, Subcommittee SC 9, Microbiology Annex A of this International Standard is for information only. Cropyriat lntenaional Organization tor Standardizatin ii ed without license from IHS Not for Resale