ISO INTERNATIONAL STANDARD 10705-1 First edition 1995-08-01 ANsl Imtarnat Doc Sec Water quality Detection and enumeration of bacteriophages Part 1: Enumeration of F-specific RNA bacteriophages Qualité de I'eau -- Détection et dénombrement des bacteriophages Partie 1: Dénombrement des bacteriophages ARN F speécifiques TSO Reference number ISO 10705-1:1995(E) No reproduction or networking pemited withoutlicense from IHS Not for Resale ISO 10705-1:1995(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work of preparing International Standards is normally carried out through Iso whicha technical committeehas beenestablishedhastherighttobe represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. IsO collaborates closely with the International Electrotechnical .Commission (lEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 10705-1 was prepared by Technical Committee ISO/TC147， Water quality. Subcommittee sc 4, Microbiological methods. Is0 10705 consists of the following parts, under the general title Water quality Detection and enumeration of bacteriophages: Part 1: Enumeration of F-specific RNA bacteriophages -Part2:Enumerationof somatic coliphages Annex A forms an integral part of this part of IsO 10705. Annexes B and C are for information only. @ISO1995 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International OrganizationforStandardization Case Postale 56 . CH-1211 Geneve 20 · Switzerland Printed in Switzerland r Standardization Noreproductionornetworking pemitedwithoutlicensefromIHS Not for Resale INTERNATIONALSTANDARD ISO 1SO 10705-1:1995(E) Water quality Detection and enumeration of bacteriophages : Part 1: Enumeration of F-specific RNA bacteriophages 1 Scope tions of the standards indicated below. Members of IEc and ISO maintain registers of currently valid This part of IS0 10705 specifies a method for the International Standards. detection and enumeration of F-specific ribonucleic acid (RNA) bacteriophages by incubating the sample IS0 3696:1987, Water for analytical laboratory use with an appropriate host strain. The method can be Specification and test methods. applied to all kinds of water, sediments and sludges ISO 5667-1:1980,Water quality where.necessary after dilution. In the case of low Sampling Part 1: Guidance on the design of sampling pro- numbers, a preconcentration step may be necessary for which a separate part of IS0 10705 will be devel- grammes. oped. The method can also be applied to shellfish ISO 5667-2:1991, Water quality Sampling extracts. Depending on the relative abundance of F Part 2: Guidance on sampling techniques. specific RNA bacteriophages to background organisms, additional confirmatory tests may be ISO 5667-3:1994, Water quality necessary and are also specified in this part of Sampling Part 3: Guidance on the preservation and handling of ISO10705. samples. The presence of F-specific RNA bacteriophages in a water sample generally indicates pollution by ISO 6887:1983, Microbiology— General guidance for the preparation of dilutions for microbiological exam- wastewater contaminated by human or animal faeces Their survival in the environment, removal by widely ination. used water treatment processes and concentration Is0 8199:1988, Water quality — General guide to the or retention by shellfish resembles that of foodborne and waterborne human enteric virus