INTERNATIONAL ISO STANDARD 3401 Second edition 1991-12-01 Ask Intarnat toc stc Cigarettes --- Determination of alkaloid retention by the filters -- Spectrometric method Cigarettes -- Determination de la retention des alcaloides par les fitres -- Methode spectrometrique Reference number ISO 3401:1991(E) e with ISC Not for Resale 1SO 3401:1991(E) Foreword isO (the International Organization for Standardization) is a worldwide federation of national standards bodies (Iso member bodies). The work of preparing international Standards is normally carried out through isO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with IsO, aiso take part in the work. IsO collaborates closely with the International Electrotechnical Commission (tEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Interna- tional Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard isO 3401 was prepared by Technicat Committee Iso/TC 126, Tobacco and tobacco products. This second edition cancelsand replaces first edition (1sO 3401:1977), which has been technically revised. C ISO : 1991 All rights reserved. No part of this publication may be reproduced or utilized In any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organlzation for Standardization Case Postale 56 · CH-1211 Geneve 20 · Switzerland Printed in Switzerland Copyright International Organization for Standardization Not for Resale INTERNATIONAL STANDARD ISO 3401:1991(E) Cigarettes -- Determination of alkaloid retention by the filters -- Spectrometric method Iso 3308:1991, Routine analytical cigarette-smoking 1 Scope machine -- Definitions and standard conditions. This International Standard specifies two methods Iso 3400:1989, Cigarettes -- Determination of alka- for the spectrometric determination of alkaloid re- loids in smoke condensates tention by fiters of cigarettes: Spectrometric method. the direct method; ！ IsO 3402:1991, Tobacco and tobacco products -- At- mosphere for conditioning and testing. the indirect method. The methods are applicable to filter cigarettes. The Iso 4387:1991, Cigarettes -- Determination of total direct method shall be used unless it is not applica- and nicotine-free dry particulate matter using a rou- ble owing to incomplete recovery of the retained al- tine analytical smoking machine. kaloids from the filter material (for example, with Iso 8243:1991, Cigarettes -- Sampling some types of charcoal filters). The indirect method is not applicable to cigarettes with perforated or porous filter tipping wraps. This International Stan- dard is not applicabte in the case of filters having Definition 3 an irreversibie nicotine retention and equipped with perforated or porous wrapping. For the purposes of this International Standard, the following definition applies. These methods determine the retention only of NOTE 1 alkaioids of tobacco smoke, expressed as nicotine. The retention of other substances present in the mainstream 3.1 alkaloid retention index Rnic of a cigarette filter: smoke is not necessarily related to the alkaloid retention The ratio, expressed as a percentage by mass, of the alkaloids retained by the filter to those entering the filter: Fnic. Rnic × 100 Snic S nlc Ynt Normative references 2 S nitc The following standards contain provisions which, through reference in this text, constitute provisions of this Internationai Standard. At the time of publi- cation, the editions indicated were valid. All stan- The alkaloid retention index may be determined as dards are subject to revision, an