6639/3 International Standard (ISO INTERNATIONAL ORGANIZATION FOR STANDARDIZATIONME KAYHAPOAHAR OPIAHM3AUMA NO CTAHAAPTM3AUMM+ORGANISATION INTE RNATIONALE DE NORMALISATION Cereals and pulses - Determination of hidden insect infestation Part 3: Reference method Cereales et legumineuses -Determination de I'infestation cachee par les insectes - Partie 3: Methode de reference First edition - 1986-12-15 S06639/3-1986 (E) UDC 633.1 : 635.65 : 632.7 Ref.No.1SO 6639/3-1986 (E) Descriptors:agriculturalproducts,cereal products,leguminousgrains,tests,determination,insects,contamination,analysismethods. Price based on 4 pages Foreword ISo (the International Organization for Standardization)is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through Iso technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with ISO, also take part in the work. Draft International Standards adopted by the technical committees are circulated to the mermber bodies for approval before their acceptance as International Standards by the ISo Council. They are approved in accordance with ISO procedures requiring at least 75 % approval by the member bodies voting. International Standard ISO 6639/3 was prepared by Technical Committee ISO/TC 34, Agriculturalfoodproducts. Users should note that all International Standards undergo revision from time to time and that any reference made herein to any other International Standard implies its latest edition, unless otherwise stated. InternationalOrganizationforStandardization,1986 Printed in Switzerland Copvriaht International Oraanization for Standardizatior INTERNATIONAL STANDARD ISO6639/3-1986(E) Cereals and pulses - Determination of hidden insect infestation - Part 3: Reference method 0 Introduction 4. Principle This International Standard describes methods of determining Maintaining test samples at a controlled temperature and hidden insect infestation in cereals and pulses. It consists of the relative humidity such that the greatest possible proportion of following parts : the insects present in the sample when collected can develop to the adult stage. Removal of insects that emerge from the Part 1:General principles grains, identification and counting, at close intervals, to enable the number initially present to be identified. Part 2: Sampling. Part 3: Reference method. Part 4: Rapid methods. 5 Apparatus Usual laboratory apparatus, and in particular: 1 Scope and field of application 5.1 Airtight containers, for storage of samples prior to the determination of moisture content (see Iso 712). This part of iso 6639 specifies the reference method for deter- mining the nature and number of hidden insects in a sample of cereals or pulses. Its aim is to count all the individuals, at every 5.2 Balance, accurate to 1 g and capable of weighing at stage of life, of every insect species that normally feeds and least 300 g. developswithin cerealsandpulses. The method is slow because it allows each insect to complete 5.3 Transparent containers, preferably made of glass or its developmental cycle and to emerge as an adult from the plastic, of a size capable of holding up to 300 g of the sample to grain before it is removed. It can be used reliably for species be tested, in a layer having a depth not exceeding 50 mm. that normally feed within grains, but not for species that oc- casionally feed in holes or cracks in grains. Thesemaybe 5.4 Closures, to allow exchange of air, but to prevent shaken from the grains or be induced to leave them by the disturbance of handling at any stage of the life cycle and some insects and mites frorm entering or leaving the containers (5.3). are likely to be killed in the process. The numbers of such NOTE- Filter papers