ISO INTERNATIONAL STANDARD 6639-4 First edition 1987-02-01 INTERNATIONAL ORGANIZATION FOR STANDARDIZATION ORGANISATION INTERNATIONALE DE NORMALISATION MEXKAYHAPOAHAA OPIAHM3AMA NO CTAHAAPTM3ALMM Cereals and pulses -- Determination of hidden insect infestation - Part 4 : Rapid methods Cereales et legumineuses -- Détermination de I'infestation cachee par les insectes Partie 4: Methodes rapides Reference number ISO 6639-4 : 1987 (E) tted without license from IHS Not for Resale Foreword ISo (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with isO, aiso take part in the work. Draft International Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the iSo Council. They are approved in accordance with ISO procedures requiring at least 75 % approval by the member bodies voting. International Standard iSO 6639-4 was prepared by Technical Committee ISO/TC 34, Agricultural food products. Section five cancels and replaces IS0 1162-1975. Users should note that all International Standards undergo revision from time to time and that any reference made herein to any other Iinternational Standard implies its latest edition, unless otherwise stated. International Organization for Standardization, 1987 Printed in Switzerland anadza Not for Resale ISO 6639-4 : 1987 (E) INTERNATIONAL STANDARD Cereals and pulses -- Determination of hidden insect infestation Part 4 : Rapid methods O Introduction Section two : Ninhydrin method (clauses 10 to 16) This International Standard describes methods of determining insect infestation, particularly wheat, sorghum, rice and similar hidden insect infestation in cereals and pulses. It consists of the following parts : sized grains. Large grains, such as maize, have to be partially broken (kibbled) before they can be tested. This treatment of Part 1: General principles. large grains can cause some insects to be lost or fragmented, thus rendering the interpretation of results unreliable. Numbers Part 2: Sampling. of eggs and young larvae may be underestimated, but, in this Part 3: Reference method. respect, the method is no less efficient than any other. Part 4: Rapid methods. Section three : Whole grain flotation method (clauses 17 to 24) Scope and field of application The method is suitable for detecting hidden infestation in most This part of ISo 6639 specifies five rapid methods for cereals and pulses but only on a qualitative basis. estimating the degree of, or detecting the presence of, hidden insect infestation in a sample of a cereal or pulse. Section four: Acoustic method (clauses 25 to 31) NOTE --- The characteristics leading to the choice of rapid method are The method is suitable for detecting living insect adults and summarized in the table in ISO 6639/1. larvae feeding inside grains. It does not permit dead adults and larvae or living eggs and pupae (non-feeding stages) to be Section one : Method by determination of carbon dioxide detected. production (clauses 3 to 9) The method is primarily intended for testing whole grains. It is Section five: X-ray method (clauses 32 to 38) not applicable for testing The method is suitable for detecting living and dead larvae and adults within grains. Insects which have been recently killed a) finely ground grain products, as there is a risk that par- ticles of material will be sucked up with air samples; or (for example by fumigation) may be difficult to distinguish from those still living. b) grain products with moisture contents greater than 15 % (m / m), because of